VARIASI DOSIS KAPORIT UNTUK DESINFEKSI Bacillus subtilis PADA LIMBAH BOTOL INFUS RS PKU MUHAMMADIYAH KOTA YOGYAKARTA

2019-11-18

en

Thesis

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Background of study: Minister of Health Regulation No. 1204 of 2004 about the requirement of hospital environmental health states that solid medical waste will be reused have to go through a chemical disinfection process with Bacillus subtilis. The solid medical waste will be reused is drip waste bottle. Chemical disinfection to be carried out using chlorine which contains a chlorine compound with a concentration of 60%. According to previous researcher conducted at PKU Muhammadiyah Hospital in Yogyakarta found Bacillus subtilis in the drip waste bottle. Aim of study: This research aims at removing Bacillus subtilis found in the drip waste bottle in PKU MuhammadiyahYogyakarta Hospital by using chlorine as the chemical disinfection. Research Method: This research used Experiment with Post Test only with control design as the method. This research was conducted in December 2018 to May 2019. The sample of this study was the drip waste bottle of PKU Muhammadyah Yogyakarta Hospital. The data analysis used descriptive with negative and positive results to find out the effectiveness dose of chlorine variationas the disinfection material. Result: The variation dose of chlorine was used in the disinfection included 0.25 ppm, 0.5 ppm, and 0.75 ppm. Descriptive test results indicated that 0.25 ppm soaking chlorine shown 0% negative Bacillus subtilis, 0.5 ppm shown 50% negative Bacillus subtilis, 0.75 ppm shown 66.7% negative Bacillus subtilis, and the group of control shown 0% negative Bacillus subtilis. Conclusion: From the finding, it can be concluded that the dose variation have not been able to kill 100% of Bacillus subtilis.