UJI HOMOGENITAS DAN STABILITAS SERUM SAPI DENGAN PENGGUNAAN PENGAWET NaN3 2% YANG DISIMPAN PADA SUHU -20°C SEBAGAI ALTERNATIF SERUM KONTROL TERHADAP KADAR TOTAL PROTEIN

2018-12-27

en

Thesis

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Background: Internal quality assurance of clinical laboratory is done to ensure the quality of laboratory examination. One of the activities of internal quality assurance in clinical chemistry laboratories is quality control that requires control serum. Research says that the use of control materials from serum animals such as cow and horse is more recommended because animal serum is free of infectious diseases such as HIV, HBV, and HCV. One of the quality control parameters in clinical laboratory is total protein examination. Objective: To determine the results of homogeneity and stability tests of cow serum with the use of NaN3 preservatives at 2% stored at -20°C to total protein levels. Methods: This study is a quasi-experimental using Pre and Post Test design. The object of this research is cow blood taken from Segoroyoso Slaughterhouse, Imogiri, Bantul. Data analysis using homogeneity and stability tests were calculated statistically based on ISO 13258:2005. Results: The homogeneity test data obtained a value of 0.3 σ is 0.87876, then the sample is declared homogeneous because it meets the criteria Ss ≤ 0.3 σ which is 0.04784 ≤ 0.87867. Based on homogeneity test data obtained Xr = 7.93000 and stability test data obtained Yr = 7.75 so that |Xr – Yr| = |7.93000 – 7.75| = 0.19, the price of 0.3 σ = 0.87867, then the sample is declared stable because it meets the criteria |Xr – Yr| ≤ 0.3 σ which is 0.19 ≤ 0.87867. Conclusion: Cow serum that have been stored for 10 weeks at -20°C are considered homogeneous and stable to total protein levels by meeting the criteria in ISO 13528:2005. Keywords: Homogeneity, stability, cow serum, total protein levels