PENGARUH LAMA PENYIMPANAN PLASMA LITHIUM HEPARIN PADA SUHU RUANG BER-AC (25±1°C) TERHADAP KADAR BILIRUBIN TOTAL

2022-05-23

en

Thesis

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Background: Examination of total bilirubin is one of the tests to determine liver function. The quality of total bilirubin examination results can be influenced by preanalytic factors such as storage. The increase in plasma heparin storage time causes a change in the sample which causes a decrease in the total bilirubin level. Research Objectives: To determine the effect of plasma lithium heparin storage at a temperature of 25±1ºC on total bilirubin levels Research Methods: The type of research used is a pre-experiment with a posttest only design. This study used lithium heparin plasma samples which were divided into 4 treatment groups, namely storage at a temperature of 25±1ºC for 0 hours (immediate examination), 4 hours, 8 hours and 24 hours. The data obtained were analyzed statistically by data normality test and Repeated Measures ANOVA test. Results: The results of this study indicate that there are differences in total bilirubin levels in lithium heparin plasma with variations in storage time at 25±1ºC based on the statistical analysis of Repeated Measures ANOVA. The percentage decrease in total bilirubin examination results in lithium heparin plasma stored for 4 hours, 8 hours and 24 hours, respectively, was 2.7%, 8.1% and 18.9%. Further test results showed that the storage time of plasma lithium heparin for 24 hours had a significant effect on reducing total bilirubin levels. Conclusion: There is an effect of plasma lithium heparin storage at a temperature of 25±1ºC on total bilirubin levels. The examination experienced a decrease in total bilirubin levels along with an increase in plasma lithium heparin storage time at a temperature of 25±1ºC Keywords: storage time, plasma lithium heparin, total bilirubin levels