Utilization of Rice Bran (Oryza Sativa L.) Situ Bagendit Variety as an Alternative Media for Fungal Growth Trichophyton Mentagrophytes

Poltekkes Kemenkes Yogyakarta

2021-12-31

eng

info:eu-repo/semantics/article

application/pdf

Identification of fungi requires culture or propagation through a growth medium. Media commonly used in Potato Dextrose Agar (PDA) including instant media made by factories or companies in ready-to-use form, is expensive and can only be found in certain places so an alternative medium that is easier to make and easy to obtain is rice bran media (Oryza sativa L.) Situ Bagendit variety. The utilization of rice bran as a growth medium for microorganisms is based on the nutritional components needed by the microorganisms.  Rice bran (Oryza sativa L.) Situ Bagendit variety can be used as an alternative medium for the growth of Trichophyton mentagrophytes, the average diameter of growth of fungal colonies on rice bran media and PDA media, the effectiveness of fungal colony growth on rice bran media compared to PDA. Pre-experimental research with Static Group Comparison research design. Research subjects Trichophyton mentagrophytes with the research object of rice bran (Oryza sativa L.) Situ Bagendit variety. The results of measuring the diameter of the Trichophyton mentagrophytes colony on rice bran media mean 75.77 mm, and the average colony diameter on PDA media is 75.52 mm. The difference in the mean colony diameter in rice bran media compared to PDA media was 0.25 mm or 0.33%. The effectiveness of growth is very effective.  Rice bran (Oryza sativa L.) Situ Bagendit variety can be used as an alternative medium for the growth of Trichophyton mentagrophytes with a 10% concentration.